| Autor: |
Tulsook, Kan, Bussadee, Piyada, Arnthong, Jantima, Mhuantong, Wuttichai, U-thai, Panida, Trakarnpaiboon, Srisakul, Champreda, Verawat, Suwannarangsee, Surisa |
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| Zdroj: |
Bioresources & Bioprocessing; 10/18/2024, Vol. 11 Issue 1, p1-12, 12p |
| Abstrakt: |
Trehalose production via a one-step enzymatic route using trehalose synthase (TreS) holds significant promise for industrial-scale applications due to its simplicity and utilization of low-cost substrates. However, the development of a robust whole-cell biocatalyst expressing TreS remains crucial for enabling practical and economically viable production. In this study, a high-sugar tolerant strain of S. cerevisiae was screened and employed as a host cell for the cell surface display of TreS from Acidiplasma aeolicum. The resultant strain, S. cerevisiae I3A, exhibited remarkable surface displayed TreS activity of 3358 U/g CDW and achieved approximately 64% trehalose yield (10.8 g/L/h productivity) from maltose. Interestingly, no glucose by-product was observed during trehalose production. The S. cerevisiae I3A cells exhibited reusability for up to 12 cycles leading to potential cost reduction of trehalose products. Therefore, our study demonstrated the development of a high-sugar tolerant S. cerevisiae strain expressing TreS on its surface as a whole-cell biocatalyst for efficient and economical trehalose production with potential applications in the food and pharmaceutical industries. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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