| Abstrakt: |
Biosynthesized silver nanoparticles (Ag-NPs) hold great potential for preventing, treating, diagnosing, and controlling diseases. The primary objective of this study is on marine halophilic bacteria (Marinobacter litoralis CARE-V18) and their extracellular synthesis of Ag-NPs. The marine bacterial synthesized Ag-NPs is characterized using dynamic light scattering (DLS), zeta potential analysis (ZPA), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The antibiofilm and antibacterial activity of the bacterially synthesized Ag-NPs against human bacterial pathogens are tested (A. baumannii, K. pneumonia, and MRSA). Furthermore, the acute toxicity effects of Ag-NPs in zebrafish embryos (ZFE) (Danio rerio) are also examined in this study. The acute toxicity of Ag-NPs at different time points of post-fertilization (3, 12, 24, 48, 72, and 96 h) eggs of zebrafish. Results shows dose-dependent antibacterial and antibiofilm activity, with significant inhibition observed at 100 and 200 µg/ml concentrations against A. baumannii, K. pneumonia, and MRSA. Thelethal concentration (LC50) values determined as 12.5 and 50 µg/ml. The acute toxicity of Ag-NPs treated ZFE are displayed concentration-dependent mortality rates and increased hatching delay. [ABSTRACT FROM AUTHOR] |
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