Renal Sugar Metabolites and mRNA Expression of Glucose Transporters in Meat-Type Chickens with Differing Residual Water Intake.

Autor: Milfort, Marie C., Ghareeb, Ahmed F. A., Ariyo, Oluwatomide W., Kwakye, Josephine, Hartono, Evan, Sovi, Selorm, Aryal, Bikash, Fuller, Alberta L., El Sabry, Mohamed I., Stino, Farid, Rekaya, Romdhane, Aggrey, Samuel E.
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Zdroj: Animals (2076-2615); Oct2024, Vol. 14 Issue 19, p2912, 11p
Abstrakt: Simple Summary: It is vitally important that methods to combat climate change-induced effects on water availability are studied in agriculture. We have previously determined the voluntary water usage of modern broilers and, in this article, further explore the molecular differences between meat-type chickens that consume more or less water than average. We show here that sugar metabolites and the expression of glucose transporter genes in the kidneys are different between these birds. These differences can aid in the selection of birds that drink less water than average for the same feed intake and body weight gain. This could help reduce the use of water in the poultry industry. Molecular differences exist between birds with high residual water intake (HRWI) compared to those with low residual water intake (LRWI). Residual water intake (RWI) is defined as the difference between the water intake of a bird and the expected water intake corrected for metabolic body weight, feed intake, and body weight gain. Tissue metabolomic analysis revealed significantly increased kidney glucose, fructose, and arabitol in the LRWI group compared to the HRWI group. mRNA expression analysis of apical sodium glucose cotransporters SGLT1, SGLT4, SGLT5, and SGLT6 showed decreased expression of SGLTs 1, 5, and 6 in LRWI birds (p < 0.05), whereas SGLT4 expression was increased compared with HRWI birds (p < 0.01). An analysis of basal glucose transporters GLUT1, GLUT2, GLUT5, and GLUT9 showed significantly increased GLUT2 expression in LRWI birds compared with HRWI birds (p < 0.01). We postulate that SGLT4 is the main apical transporter in chicken kidneys and that its increased expression reduces these birds' need for water, resulting in less drinking. This is balanced by the increased expression of the basal transporter GLUT2, indicating better glucose retention, which may partly explain the physiological mechanism behind why these birds drink less water. Innately driven broiler water intake could therefore be influenced by the expression of kidney solute transporters. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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