| Autor: |
Vazquez-Avendaño, José Roberto, Cortez-Romero, César, Ambríz-García, Demetrio Alonso, Rodríguez-Suástegui, José Luis, Hernández-Pichardo, José Ernesto, Navarro-Maldonado, María del Carmen |
| Předmět: |
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| Zdroj: |
Animals (2076-2615); Oct2024, Vol. 14 Issue 19, p2894, 13p |
| Abstrakt: |
Simple Summary: The in vitro culture of mammalian embryos lacks different embryotrophic factors that are present in the female reproductive tract and are essential for correcting embryonic development. It has been reported that supplementation with oviductal fluid (OF) and uterine fluid (UF) during the in vitro culture of mammalian embryos produced by IVF has a positive effect on their development and quality. We investigated the effect of OF–UF supplementation on the balance of reactive oxygen species (ROS), glutathione (GSH), and the development of cloned and parthenogenetic Ovis aries embryos. However, at concentrations of 1 and 2% OF–UF, the blastocyst rate decreased in both groups of embryos. At a concentration of 1% OF–UF, both intracellular ROS and GSH decreased in the blastocysts of parthenogenetic embryos but not in cloned embryos. In cloned sheep embryos, OF–UF had no impact on the embryonic development or redox balance. Somatic cell nuclear transfer (SCNT) has great potential for the replication of high-commercial-value animals, threatened wild species for conservation purposes, and transgenic animals for biomedical purposes. However, SCNT has a low success rate due to intrinsic factors of the technique itself, which leads to low rates of embryonic development and epigenetic alterations in cloned embryos. The objective of this study was to evaluate the effect of OF–UF on the intracellular concentrations of ROS and GSH and the development of cloned and parthenogenetic Ovis aries embryos. The results do not show a beneficial effect on the development of parthenogenetic and cloned embryos at concentrations of 0.5% OF–UF. Furthermore, at 1% OF–UF, an adverse effect was observed in cloned embryos at the blastocyst stage and 2% OF and UF in parthenogenetic embryos during the first divisions. Decreases in ROS and GSH levels were observed in the parthenogenetic blastocysts treated with 1% OF–UF, but not in the clones, in which a higher concentration of GSH and a similar concentration of ROS were observed. No effect of OF–UF was observed on embryonic development and redox balance in sheep embryos cloned via handmade cloning. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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