| Abstrakt: |
In order to analyze the high-temperature Daqu bacterial taxa and their physicochemical properties in Linyi area, Shandong Province, Illumina MiSeq high-throughput sequencing technology and pure culture technology were used to analyze the bacterial taxa of high-temperature Daqu in Lanling wine, and then the physicochemical indexes were determined by conventional detection methods in this study. Finally, the correlation between bacterial taxa and physicochemical indexes in Daqu was explored. The results showed that the main genera of bacteria in Daqu were Kroppenstedtia, Thermoactinomyces, Saccharopolyspora, Staphylococcus, Rhodococcus, Weissella and Bacillus, et al. Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans were cultureable dominant bacterial isolates. There was a large difference in saccharification, esterification, alcoholization and liquefaction between the 15 parts of high-temperature Daqu. Kroppenstedtia, Staphylococcus, Leucobacter, Rhodococcus and Weissella were significantly positively correlated with esterification, fermentation, alcoholization, esterification and liquefaction (P<0.05). Thus, there are a large number of core bacterial taxa in high-temperature Daqu in Lanling wine, and there is a significant correlation between various bacterial genera and physicochemical indexes, which further provides a reference for the improvement and enhancement of the quality of high-temperature Daqu in this area. [ABSTRACT FROM AUTHOR] |
