Abstrakt: |
In this study, we evaluated the antimicrobial, antibiofilm and antioxidant properties, in addition to the chemical constituents of Satureja graeca L. ethyl acetate and n-butanol fractions using HPLC-DAD analysis, phenolic compound detection was established. Six in vitro assays (DPPH, ABTS, β-carotene, O2•-, CUPRAC, and Reducing power) were employed to assess the antioxidant capacities. In order to determine the minimum inhibitory concentrations (MIC) of both extracts against six bacterial strains and two fungi, the serial micro-dilution method was used. According to analysis, the extracts' main phenolic components were chlorogenic acid, rutin, ellagic acid, vanillin, and caffeic acid. Both fractions revealed high antioxidant capacities at different levels in all assays and they showed a strong antimicrobial activity against all tested strains as MIC came out to be 0.3-10 mg/ml. However, ethyl acetate fraction exerted important effect compared to that of n-butanol fraction on Enterococcus faecalis ATCC19433 (1.2 and 2.5 mg/mL), Candida albicans ATCC10239 (2.5 and 10 mg/mL) and Candida tropicalis RSKK 665 (2.5 and 5 mg/mL). Furthermore, ethyl acetate fraction showed a significant antibiofilm activity against C. tropicalis RSKK 665 (41.9%) and C. albicans ATCC 10239 (38.6%) at 2.5 mg/mL. These findings suggest that Satureja graeca L. can be used in food and may be a promising therapeutic agent for treating a wide range of disorders. [ABSTRACT FROM AUTHOR] |