| Autor: |
Wei, Ziyao, Zhang, Yuyue, Yuan, Yuru, Li, Lu, Li, Tingjin, Guan, Yulu, Wang, Di, Gao, Yimeng, Gao, Qingsong, Ji, Jianhui, Nguyen, Thanhliem, Liu, Xi |
| Zdroj: |
Journal of Plant Biology; Oct2024, Vol. 67 Issue 5, p409-418, 10p |
| Abstrakt: |
Rice is a very important food crop: two-thirds of the population of the world depend on rice as a staple food. Furthermore, since grain size has great influence on rice yield and quality, it is important to study the regulatory genes and mechanisms related to grain size to help cultivate high-yield and high-quality rice varieties. In this study, rice ubiquitin-conjugated enzyme (UBC) gene OsUBC16 was been identified, cloned, and analyzed. The analysis showed that this gene has a negative regulatory effect on grain weight. Specifically, OsUBC16 knockout mutants produced short grain length, large grain width, and increased 1000-grain weight. Scanning electron microscopy showed that the epidermal cells of the mutants seed changed in size as well, and OsUBC16 was highly expressed in seeds and panicles. Subcellular localization revealed that OsUBC16 was located in both the cytoplasm and the nucleus. In addition, OsUBC16 had high homology with the related proteins of maize, Arabidopsis, and soybean. Yeast two-hybrid and bimolecular fluorescence complementation experiments showed that OsUBC16 interacts with Heading and Grain Weight (HGW), a ubiquitin-associated (UBA) domain protein. In conclusion, OsUBC16 may regulate rice grain size and weight through interaction with HGW, a finding that lays a foundation for elucidating the molecular mechanism of OsUBC16 in regulating rice grain size, and providing gene resources for the high-yield molecular breeding of rice. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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