| Abstrakt: |
Introduction: Mitophagy is a multifunctional pathway that can lead to mitochondrial defects or greater efficiency in skeletal muscles. Physical activities are an important factor in the regulation of mitophagy according to conditions, such as intensity, duration, and type. Therefore, the current study aimed to investigate the effect of moderate-intensity interval training (MIIT) on the intracellular content of proteins related to the mitophagy (FUNDC1 and NIX) pathway in the soleus muscle of male Wistar rats. Methods: In this experimental study, 16 three-month-old Wistar rats with an average weight of 280±30 were randomly divided into two Control and MIIT groups (8 rats per group). Intervention for rats in the MIIT group consisted of running on a treadmill for 8 weeks, 5 sessions per week, and each session consisted of 10 MIIT bouts of 3 minutes, separated by 2-minute rest periods. In the first week, the average speed was 19 m/min, and in the last week, it reached 25 m/min. The variables were measured using the Western Blotting laboratory technique in the soleus muscle tissue. Data were analyzed using an independent t-test with GraphPad Prism software version 9.5. A significance level of p≥0.05 was considered. Results: The content of FUNDC1 and NIX proteins showed a significant decrease in the MIIT group compared to the control group (P<0.0001). Conclusion: Considering the reduction of these factors, MIIT training can regulate the mitophagy process in skeletal muscles and help maintain mitochondrial health through related pathways. Therefore, further research is required. [ABSTRACT FROM AUTHOR] |
