Characterization of macrophages associated with human skin models exposed to UV radiation.

Autor: Phuphanitcharoenkun, Suphanun, Louis, Fiona, Sowa, Yoshihiro, Uchida, Kentaro, Katsuyama, Misa, Waditee-Sirisattha, Rungaroon, Kageyama, Hakuto, Matsusaki, Michiya, Palaga, Tanapat
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Zdroj: Communications Biology; 10/8/2024, Vol. 7 Issue 1, p1-13, 13p
Abstrakt: Skin macrophages play important roles in the response to external stimuli. Human skin equivalents (HSEs) incorporating the human monocytic cell line THP-1 were fabricated to generate immunocompetent human skin models. These HSEs were used to investigate the influence of the skin microenvironment and ultraviolet A (UVA) on macrophages. Transcriptomic analysis revealed that THP-1 cells in HSEs were enriched in extracellular matrix interaction hallmark but downregulated in DNA replication hallmark. Upon UVA exposure, immunocompetent HSEs presented epidermal distortion and increased DNA double-strand breaks (DSBs). The genes associated with oxidative stress and the inflammatory response were significantly upregulated in THP-1 cells. When the photoprotective agent mycosporine-2-glycine from cyanobacteria was applied to HSEs, the incidence of UVA-induced DSBs was significantly lower, and inflammatory and UV response hallmarks were downregulated in THP-1 cells. Taken together, these results suggest that immunocompetent HSEs can be used to investigate the responses of skin-resident macrophages to external stimuli. Human skin equivalents respond to UV stimulation by upregulating inflammatory and oxidative stress pathways in macrophages, representing a model to study skin immune responses to external stimuli. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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