| Autor: |
Valero-Rivera, Karla Paulina, Magaña-Aquino, Martin, Romero-Méndez, María del Carmen, Morales-Barragán, Miriam Nayeli, Sagahón-Azúa, Julia, Medellín-Garibay, Susanna Edith, Milán-Segovia, Rosa del Carmen, Martínez-Gutiérrez, Fidel, Romano-Moreno, Silvia |
| Předmět: |
|
| Zdroj: |
Journal of Chromatographic Science; Sep2024, Vol. 62 Issue 8, p783-788, 6p |
| Abstrakt: |
A sensitive and rapid ultra-performance liquid chromatography coupled with -tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine ceftibuten (CTB) and sulbactam (SUL) in human plasma. An ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm column with gradient elution of water (0.1% formic acid) and acetonitrile was used for separation at a flow rate of 0.2 mL/min. This method involves a simple sample preparation with acetonitrile. The calibration curves of CTB and SUL in plasma showed good linearity over the concentration range of 0.50–25 μg/mL and with a correlation coefficient (r 2) >0.99. This method was validated in terms of selectivity, linearity, precision, accuracy and stability. High precision was obtained with coefficients of variation <15%. Excellent recovery in the range of 90–104% was achieved for CTB and SUL was 86–110%. The method has the potential utility to support pharmacometric modeling in clinical practice and biopharmaceutic studies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
