| Autor: |
Saheb Sharif-Askari, Fatemeh, Zakri, Adel M., Alenazy, Maha Fahad, El-Wetidy, Mohammed S., Khalid Salah Al-Sheakly, Baraa, Saheb Sharif-Askari, Narjes, ALKufeidy, Roua M., Omair, Mohammed A., Al-Muhsen, Saleh, Halwani, Rabih |
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| Zdroj: |
Inflammation Research; Oct2024, Vol. 73 Issue 10, p1699-1709, 11p |
| Abstrakt: |
Aims: This study aimed to investigate the effect of interleukin-35 (IL-35) on inflamed lung tissue in a murine model of asthma. IL-35 was examined for its potential to induce regulatory lymphocytes during ovalbumin (OVA)-induced acute lung injury. Methods: Female BALB/c mice sensitized with OVA and were treated with recombinant IL-35 (rIL-35) via intranasal or intraperitoneal routes and were administered 4 h before OVA challenge. The effects of rIL-35 treatment on the lung and blood levels of regulatory B cells (Bregs) and regulatory T cells (Tregs), as well as their production of immunosuppressive cytokines, were determined using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively. Results: Treatment of OVA-sensitized asthmatic mice with rIL-35, whether administered intranasally or intraperitoneally, resulted in reduced lung inflammation and injury. This reduction was accompanied by an increase in the frequency of IL-35 producing Bregs, IL-35 and IL-10 producing Bregs, and conventional LAG3+ Tregs in the lung tissues and blood. This increase was more pronounced with intranasal rIL-35. Furthermore, there was a positive correlation between the levels of these regulatory cells and lung gene expression of IL-35 and IL-10, and an inverse correlation with both lung gene expression and plasma level of IL-17. Conclusions: The results of this study suggest that IL-35, through its ability to increase Bregs and Tregs, is effective in reversing lung inflammation in the context of asthma. Since the increase was more pronounced with intranasal administration, this highlights the therapeutic potential of its local intrapulmonary application in managing asthma-related inflammation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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