Assessing the Potential of Tortistilus (Hemiptera: Membracidae) from Northern California Vineyards as Vector Candidates of Grapevine Red Blotch Virus.

Autor: Hoyle, Victoria J., McGinnity Schneider, Elliot J., McLane, Heather L., Wunsch, Anna O., Fendell-Hummel, Hannah G., Cooper, Monica L., Fuchs, Marc F.
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Zdroj: Insects (2075-4450); Sep2024, Vol. 15 Issue 9, p664, 15p
Abstrakt: Simple Summary: Tortistilus and Spissistilus are two genera of cryptic treehoppers from the Ceresini tribe. S. festinus is a vector of grapevine red blotch virus (GRBV) in vineyards in northern California, USA; however, the transmission capabilities of Tortistilus spp. are unknown. In this study, we determined the species of Tortistilus found in vineyards in Napa Valley, California, as T. wickhami, and determined that only a few specimens but no dissected heads with salivary glands tested positive for GRBV in PCR and qPCR. These results suggested that T. wickhami is an unlikely vector of GRBV in northern California vineyards. Ceresini treehoppers are present in northern California vineyard ecosystems, including the closely related Spissistilus and Tortistilus (Hemiptera: Membracidae). These membracids are not direct pests of wine grapes, but S. festinus is a vector of grapevine red blotch virus (GRBV). No information is available on the ability of Tortistilus spp. to transmit GRBV. In this study, Tortistilus were collected on yellow panel cards across 102 vineyard sites and surrounding areas in Napa Valley, California, USA in 2021–2023. Specimens were morphotyped, sexed and tested for GRBV ingestion and acquisition by multiplex PCR or qPCR. Phylogenetic analysis of the partial sequence of mt-COI and ITS gene fragments of a subset of 40 Tortistilus specimens revealed clustering in a monophyletic clade with T. wickhami with the former barcode sequence. Only 6% (48/758) of the T. wickhami tested positive for GRBV, but none of the heads with salivary glands (0%, 0/50) of the dissected specimens tested positive for GRBV, indicating no virus acquisition. In contrast, half of the dissected heads with salivary glands of S. festinus (52%, 12/23), from the same collection vineyard sites, tested positive for GRBV. Together, our findings confirmed the presence of T. wickhami in northern California vineyards and suggested a dubious role of this treehopper as a vector of GRBV. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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