| Autor: |
Dong, Zhimin, Li, Cheng, Tian, Xiangxue, Guo, Xiaoran, Li, Xiuli, Ren, Weike, Chi, Jingjing, Zhang, Li, Li, Fuqiang, Zhu, Yao, Zhang, Wanjiang, Yan, Minghua |
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| Zdroj: |
Veterinary Sciences; Sep2024, Vol. 11 Issue 9, p447, 13p |
| Abstrakt: |
Simple Summary: As a zoonotic pathogen, the exploration of new virulence-related genes and the functional identification of Streptococcus suis are of positive significance in revealing the pathogenic mechanism of the strain and developing new vaccines. This study provides evidence that the sugC gene is a virulence-related gene of the Streptococcus suis serotype 2 strain and plays a crucial role in regulating its adhesion and invasion. The sugC gene is a coding gene for the ATP-binding transporter-associated protein, which has been reported to exist in the highly virulent Streptococcus suis. In order to reveal the effect of the sugC gene on the virulence of Streptococcus suis serotype 2, TJS75, a wild-type strain, was used as a parent strain, and a knockout sugC strain and complementary strain were successfully constructed via homologous recombination technology. We found that the adhesion and invasion abilities of the knockout sugC strain in PK-15 cells were decreased. Most importantly, the knockout of the sugC gene could reduce the incidence rate, mortality and LD50 value of TJS75 in BALB/c mice. These results provide a reference for us to further study the pathogenic mechanism of Streptococcus suis. The sugC gene of Streptococcus suis (S. suis) is a coding gene for the ATP-binding transporter-associated protein with strong pathogenicity. In order to reveal the effect of the sugC gene on the virulence of S. suis serotype 2, a wild-type strain of TJS75, isolated from fattening pigs' brain tissue samples, was used as a parent strain, and a knockout sugC gene (ΔsugC) and complementary strain (CΔsugC) were successfully constructed via homologous recombination technology. The biological characteristics of TJS75, ΔsugC and CΔsugC were compared and analyzed through growth curves, biochemical characteristics, hemolysis characteristics, cell infection tests and pathogenicity tests on BALB/c mice. The results of the growth characteristic experiments in vitro showed that the plateau stage growth period of ΔsugC was delayed compared to the TJS75 strain, but there was no difference in the total number of bacteria. The biochemical characteristics and hemolysis ability of ΔsugC in sheep blood had no difference compared with TJS75, but its adhesion and invasion abilities in PK-15 cells were decreased. Knockout of the sugC gene had no impact on the expression levels of adhesion-related genes in TJS75 in real-time PCR analysis. In addition, the LD50 of ΔsugC in BALB/c mice was 1.47 × 108 CFU, seven times higher than that of TJS75 (LD50 = 2.15 × 107 CFU). These results illustrate that the deletion of sugC reduced the virulence of TJS75 to BALB/c mice, but its role in the adhesion and invasion of PK-15 cells in this strain needs to be further explored. In summary, this study provides evidence that the sugC gene is a virulence-related gene in the S. suis serotype 2 strain and plays a crucial role in the adhesion and invasion of S. suis. This study lays a foundation for the further exploration of the potential virulence factors and pathogenesis of S. suis. [ABSTRACT FROM AUTHOR] |
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