A novel luciferase‐based reporter tool to monitor the dynamics of carbon catabolite repression in filamentous fungi.

Autor: Rüllke, Marcel, Meyer, Franziska, Schmitz, Kevin, Blase, Hannes, Tamayo, Elisabeth, Benz, J. Philipp
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Zdroj: Microbial Biotechnology; Sep2024, Vol. 17 Issue 9, p1-18, 18p
Abstrakt: Filamentous fungi with their diverse inventory of carbohydrate‐active enzymes promise a holistic usage of lignocellulosic residues. A major challenge for application is the inherent repression of enzyme production by carbon catabolite repression (CCR). In the presence of preferred carbon sources, the transcription factor CreA/CRE‐1 binds to specific but conserved motifs in promoters of genes involved in sugar metabolism, but the status of CCR is notoriously difficult to quantify. To allow for a real‐time evaluation of CreA/CRE‐1‐mediated CCR at the transcriptional level, we developed a luciferase‐based construct, representing a dynamic, highly responsive reporter system that is inhibited by monosaccharides in a quantitative fashion. Using this tool, CreA/CRE‐1‐dependent CCR triggered by several monosaccharides could be measured in Neurospora crassa, Aspergillus niger and Aspergillus nidulans over the course of hours, demonstrating distinct and dynamic regulatory processes. Furthermore, we used the reporter to visualize the direct impacts of multiple CreA truncations on CCR induction. Our reporter thus offers a widely applicable quantitative approach to evaluate CreA/CRE‐1‐mediated CCR across diverse fungal species and will help to elucidate the multifaceted effects of CCR on fungal physiology for both basic research and industrial strain engineering endeavours. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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