Evaluation of the safety and efficacy of skin penetration enhancer Putocrin®.

Autor: Cen, Zongxiao, Chen, Zhiyuan, Wang, Ding, Chen, Xueping, Chen, Junyuan
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Zdroj: Journal of Cosmetic Dermatology; Oct2024, Vol. 23 Issue 10, p3272-3280, 9p
Abstrakt: Background: Substances that can efficiently enhance skin penetration while exerting no adverse effect are useful for drug and cosmetics formulation. Objective: To investigate the safety and enhance skin penetration efficacy of Putocrin®, a combination containing 2% isosorbide dimethyl ether, 1% pentanediol, and 0.5% inositol. Methods: An in vitro keratinocyte cell assay using 3‐(4,5‐dimethylthiazolyl‐2)‐2,5 diphenyltetrazolium bromide (MTT), and an in vitro EpiKutis® skin study adopted hematoxylin and eosin staining, immunostaining, and liquid chromatography–mass spectrometry (LC–MS) analysis were carried out to investigate the safety of Putocrin®. A pigskin‐Franz cell system experiment applied high‐performance liquid chromatography (HPLC) to compare the skin penetration efficiency of fluorescein isothiocyanate (Fitc)‐labeled tranexamic acid with or without the assistance of Putocrin®. The safety and efficacy of Putocrin® was further evaluated on zebrafish embryos. Results: The MTT assay showed that Putocrin® at concentration ≤2.5% did not significantly affect cell viability. The in vitro EpiKutis® skin study revealed that 2.5% Putocrin® did not affect skin morphology, filaggrin content, ceramide/protein, or fatty acid/protein ratios, but significantly increased loricrin content by 86.00% (p < 0.001). The pigskin‐Franz cell penetration experiment demonstrated that Fitc‐labeled tranexamic acid could barely penetrate the skin (with penetration rate of 1.121%), while Putocrin® significantly enhanced the penetration rate up to 83.983%, which was close to unlabeled tranexamic acid (90.013%). The zebrafish embryo study showed that 2.5% Putocrin® did not exert observable toxicity and obviously assisted the skin penetration of Fitc‐labeled tranexamic acid into fish embryos. These results indicate the strong enhancing skin penetration potency of Putocrin®. Conclusion: This study demonstrated the safety as well as the strong enhancing skin penetration potency of Putocrin® for cosmetics formulation use. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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