| Autor: |
Chian Teng Ong, Blackall, Patrick. J., Boe-Hansen, Gry B., deWet, Sharon, Hayes, Ben J., Indjein, Lea, Korolik, Victoria, Minchin, Catherine, Nguyen, Loan To, Nordin, Yusralimuna, Siddle, Hannah, Turni, Conny, Venus, Bronwyn, Westman, Mark E., Zhetao Zhang, Tabor, Ala E. |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology; 2024, p1-22, 22p |
| Abstrakt: |
Introduction: Bovine Genital Campylobacteriosis (BGC), caused by Campylobacter fetus subsp. venerealis, is a sexually transmitted bacterium that significantly impacts cattle reproductive performance. However, current detection methods lack consistency and reliability due to the close genetic similarity between C. fetus subsp. venerealis and C. fetus subsp. fetus. Therefore, this study aimed to utilize complete genome analysis to distinguish genetic features between C. fetus subsp. venerealis and other subspecies, thereby enhancing BGC detection for routine screening and epidemiological studies. Methods and results: This study reported the complete genomes of four C. fetus subsp. fetus and five C. fetus subsp. venerealis, sequenced using long- read sequencing technologies. Comparative whole-genome analyses (n = 25) were conducted, incorporating an additional 16 complete C. fetus genomes from the NCBI database, to investigate the genomic diglycine intolerance characteristic of C. fetus subsp. venerealis and its biovar variant. Verification with 58 annotated C. fetus genomes, both complete and incomplete, from RefSeq, successfully classified these seven SNPs into two groups, aligning with their phenotypic identification as CFF (Campylobacter fetus subsp. fetus) or CFV/CFVi (Campylobacter fetus subsp. venerealis and its biovar variant). Furthermore, we demonstrated the application of mraY SNPs for detecting C. fetus subspecies using a quantitative PCR assay. Discussion: Our results highlighted the high genetic stability of C. fetus subspecies. Nevertheless, Campylobacter fetus subsp. venerealis and its biovar variants encoded common SNPs in genes related to glycine intolerance, which di [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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