| Abstrakt: |
The present study is designed to provide a comprehensive insight into the ex situ conservation of the medicinally important endangered herb Trillium govanianum Wall. ex D. Don (Himalayan Trillium) through biotechnological tools. To prioritize ex-situ conservation, it is imperative to first understand the status of existing genetic diversity, followed by phytochemical screening to identify elite individuals or locations. Therefore, the genetic diversity of T. govanianum individuals from different locations was assessed using SSR markers. The individuals grouped into diversified clusters were further analyzed by HPLC to identify elite chemotype(s), followed by the standardization of an efficient in vitro propagation method for conservation purposes. The neighbor-joining tree and dendrogram revealed a low level of genetic diversity, thereby indicating more similarity among individuals from the same topographical environment. Based on HPLC analysis, it was revealed that at a higher elevation, the content of diosgenin was higher, which may have an impact on the accumulation of the bioactive compound (diosgenin) in T. govanianum. The rhizome bud explants harvested from the individuals of identified elite locations exhibited the maximum survival rate and shoot regeneration on MS medium enriched with BAP, Kn, and GA3. For multiplication, a substantial increase in the production of lateral buds was noticed on MS medium fortified with GA3, BAP, Kn, and casein hydrolysate. Meanwhile, higher concentrations of sucrose, growth regulators (BAP and TDZ), and additives (casein hydrolysate and L-glutamine) showed a significant effect on mini rhizome development from in vitro derived lateral buds. The in vitro regenerants were further rooted and hardened successfully. Besides, SSR and ISSR markers confirmed the genetic uniformity of in vitro raised regenerants. Thus, the study demonstrates an efficient and competent way for propagating the valuable endangered medicinal herb T. govanianum by developing in vitro mini rhizomes using rhizome bud explants. The findings will be of immense potential for its large-scale ex-situ conservation due to its narrow genetic base, along with fostering knowledge about conservation strategies.Key Message: An efficient and competent way to propagate the valuable endangered medicinal herb Trillium govanianum (Himalayan Trillium) was demonstrated by in vitro mini rhizomes production using rhizome bud explants. [ABSTRACT FROM AUTHOR] |
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