| Abstrakt: |
In recent years, chitosan oligosaccharide (COS) has demonstrated potential applications in enhancing the protective immune function of sea cucumber Apostichopus japonicus (Selenka, 1867), owing to its superior biological properties. The fluorescent probe labeling technique is simple to operate and highly sensitive, enabling the visualization of polysaccharides. This study utilized the fluorescent probe fluorescein isothiocyanate (FITC) to label COS. A fluorescence imaging system was used to track the distribution of the FITC-tagged COS absorbed by A. japonicus. FITC-COS was prepared and administered to A. japonicus along with a basal diet supplemented with 0.5% FITC-COS. Subsequently, samples were collected at different time points to analyze the differential absorption and distribution of COS in the coelomocytes and the tissues of A. japonicus, including intestinal contents, intestine, respiratory tree, longitudinal muscle, and body wall tissues. Quantitative fluorescence detection with a microplate reader and fluorescence microscopy were used for analysis. The results revealed that COS is widely distributed in the various tissues of A. japonicus. Fluorescence peaks were observed at 9.5 h in the coelomocytes, respiratory tree, longitudinal muscle, and body wall tissues. Additionally, at 12 h, fluorescence peaks were observed in the intestinal contents and intestine tissue. At the same time, the distribution of COS in the intestinal contents decreased significantly at day 3 compared to the peak value. [ABSTRACT FROM AUTHOR] |
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