| Abstrakt: |
Psoralea corylifolia, locally known as Bakuchi, is a well-known medicinal plant noted for its myriad healthcare benefits. Every part of the plant encompasses several phyto-chemical constituents whose potential application is explored by many researchers in recent times. Though profound research is done on assessment of benefits of seeds and fruits of P. corylifolia, investigation of phyto-chemical constituents and beneficial properties of leaves remained an untouched area. Therefore, the present study focused on unveiling the bio-molecules in the leaves and callus grown using leaf explants in tissue culture. The optimum concentration for callus induction utilizing NAA and BAP hormones was determined to be 5.0 mg/L NAA combined with 0.5 mg/L BAP and 5.0 mg/L NAA combined with 0.5 mg/L KN with green friable callus. Using methanol as solvent, soxhlet extractor was used to extract phyto-chemical constituents from the dried callus powder. The phyto-chemical screening of the plant extract was carried out using standard protocols. Further, anti-microbial properties against bacteria and fungi were carried out. The presence of several phyto-chemical constituents like alkaloids, steroids, terpenoids, cardiac glycosides, anthraquinone glycosides, and flavonoids in both leaves and callu was observed in the present study. Both leaves and callus shown remarkable anti-bacterial properties, especially against Staphylococcus aureus, and MDR strains such as MRSA and VRE. In addition, significant anti-fungal activity was also witnessed against Candida albicans, Candida aureus, Cryptococcus neoformans, and Cryptococcus gattii by extracts of leaves and callus. As both leaves and callus having almost similar properties, micro-propagation using tissue culture can be exploited as a potential tool in preparation of healthcare products and this is especially useful in P. corylifolia because of its slow growing nature. [ABSTRACT FROM AUTHOR] |
