| Autor: |
Yin, Weihong, Hu, Kai, Yu, Bingwen, Zhang, Tao, Mei, Haohua, Zhang, Bowen, Zou, Zheyu, Xia, Liping, Gui, Yehong, Yin, Juxing, Jin, Wei, Mu, Ying |
| Předmět: |
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| Zdroj: |
Lab on a Chip; 10/7/2024, Vol. 24 Issue 19, p4659-4668, 10p |
| Abstrakt: |
Escherichia coli O157:H7 is a major foodborne pathogen that poses a significant threat to food safety and human health. Rapid and sensitive detection of viable Escherichia coli O157:H7 can effectively prevent food poisoning. Here, we developed a microwell-confined and propidium monoazide-assisted digital CRISPR microfluidic platform for rapid and sensitive detection of viable Escherichia coli O157:H7 in food samples. The reaction time is significantly reduced by minimizing the microwell volume, yielding qualitative results in 5 min and absolute quantitative results in 15 min. With the assistance of propidium monoazide, this platform can eliminate the interference from 99% of dead Escherichia coli O157:H7. The direct lysis method obviates the need for a complex nucleic acid extraction process, offering a limit of detection of 3.6 × 101 CFU mL−1 within 30 min. Our results demonstrated that the platform provides a powerful tool for rapid detection of Escherichia coli O157:H7 and provides reliable guidance for food safety testing. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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