Dietary analysis of wolf (Canis lupus) – a comparison of markers and methods.

Autor: Eusemann, Pascal, Rees, Jana, Kuhlenkamp, Vivian, Lippitsch, Paul, Schumann, Heiner
Zdroj: Conservation Genetics Resources; Sep2024, Vol. 16 Issue 3, p217-220, 4p
Abstrakt: Metabarcoding is emerging as an alternative to morphological methods in noninvasive carnivore diet analysis based on scats. A number of metabarcoding markers have been developed but their comparative performance to recover DNA from scats remains mostly untested. We tested three markers covering a wide taxonomic range of prey items and compared them with the results of a morphological analysis. Morphological and genetic methods performed comparably regarding the identity of detected prey species, but the number of identified species varied strongly between markers. Only one, 12S-V5, amplified successfully in all samples and proved to be robust and reliable when working with the highly degraded DNA obtained from scats. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: