Introduction of a spectrophotometric method for salivary iodine determination on microplate based on Sandell-Kolthoff reaction.
| Autor: | Oblak, Adrijana, Imperl, Jernej, Kolar, Mitja, Marolt, Gregor, Krhin, Blaz, Zaletel, Katja, Gaberscek, Simona |
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| Předmět: |
SALIVA analysis
IODINE analysis COST control CAFFEINE DATA analysis T-test (Statistics) DESCRIPTIVE statistics OXIDIZING agents INORGANIC compounds MASS spectrometry RESEARCH methodology STATISTICS COMPARATIVE studies CONFIDENCE intervals DATA analysis software BIOMARKERS KIDNEYS REGRESSION analysis |
| Zdroj: | Radiology & Oncology; Sep2024, Vol. 58 Issue 3, p357-365, 9p |
| Abstrakt: | Iodine is an essential element for the synthesis of thyroid hormones. Therefore, a reliable marker of iodine supply is important. Iodine is predominantly excreted via kidneys, but also via salivary glands. Our aim was to introduce a new and simple method for determination of salivary iodine concentration (SLIC). Self-prepared chemicals and standards for Sandell-Kolthoff reaction on microplate with ammonium peroxydisulfate (AP) in the range 0−400 µg/L were used. Suitability of water-based standards (WBS) and artificial saliva-based standards (ASS) for standard curve were tested. We followed standards for method validation, defined concentration of used AP and compared our results with Inductively Coupled Plasma Mass Spectrometry (ICP-MS). WBS gave more reliable results than ASS as an underestimation of iodine concentration was found for ASS. LoB was 6.5 µg/L, LoD 12.0 µg/L, therefore analytical range was 12−400 µg/L. Intra- and inter-assay imprecisions at iodine concentrations, namely 20, 100, 165, and 350 µg/L were 18.4, 5.1, 5.7, and 2.8%, respectively, and 20.7, 6.7, 5.1, and 4.3%, respectively. Suitable molarity of AP was 1.0 mol/L and showed no difference to 1.5 mol/L (P values for samples with concentration 40, 100, and 150 µg/L, were 0.761, 0.085, and 0.275, respectively), whereas there was a significant change using 0.5 mol/L (P<0.001). Saliva samples could be diluted up to 1:8. There was no interference of thiocyanate and caffeine up to 193.5 mg/L. Our original method was comparable to ICP-MS. Spaerman coefficient was 0.989 (95% CI: 0.984−0.993). The new method for SLIC determination is in excellent agreement with ICP-MS and easy-to-use. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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