Mesenchymal Stem Cell–Conditioned Media Modulate HUVEC Response to H2O2: Impact on Gene Expression and Potential for Atherosclerosis Intervention.

Autor:	Alqasoumi, Abdulmajeed, Alsharidah, Mansour, Mahmood, Amer, Elsafadi, Mona, Al Rugaie, Osamah, Mohany, Khalid M., Al-Regaiey, Khalid A., Alyahya, Khaleel I., Alanteet, Alaa A., Algarzae, Norah K., AlGhibiwi, Hanan K., AlHomaidi, Adel, Abumaree, Mohammad, Maioli, Margherita
Předmět:	RESEARCH funding MESENCHYMAL stem cells CELL proliferation CELL physiology ATHEROSCLEROSIS CELL motility REVERSE transcriptase polymerase chain reaction OXIDATIVE stress CELLULAR therapy CULTURE media (Biology) GENE expression GENES ENDOTHELIAL cells UMBILICAL veins CELL survival
Zdroj:	BioMed Research International; 7/17/2024, Vol. 2024, p1-10, 10p
Abstrakt:	Background: We studied the potential of human bone marrow–derived mesenchymal stem cell conditioned media (hBMSC CM) in protecting endothelial cell properties (viability, proliferation, and migrations) from the deleterious effects produced by the inflammatory environment of H2O2. Additionally, we investigated their impact on the endothelial cells' gene expression of some inflammatory‐related genes, namely, TGF‐β1, FOS, ATF3, RAF‐1, and SMAD3. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured individually under three conditions: alone, with varying concentrations of H2O2, or with varying concentrations of H2O2 and hBMSC CM. HUVEC adhesion, proliferation, and migration were evaluated using the xCELLigence system. The HUVECs' gene expressions were evaluated by real‐time polymerase chain reaction (RT–PCR). Results: Generally, we observed enhanced HUVEC viability, proliferation, and migration when cultured in media supplemented with H2O2 and hBMSC CM. Furthermore, the CM modulated the expressions of the studied inflammatory‐related genes in HUVECs, promoting a more robust cellular response. Conclusion: This study has illuminated the protective role of hBMSC CM in mitigating the damaging effects of H2O2 on endothelial cell function. Our data demonstrate that hBMSC CM enhances the viability, proliferation, and migration of HUVECs even under oxidative stress conditions. Additionally, the conditioned medium was found to modulate the gene expression of pivotal markers related to inflammation, suggesting a favorable influence on cellular response mechanisms. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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