Histological differences related to autophagy in the minor salivary gland between primary and secondary types of Sjögren's syndrome.

Autor:	Ono-Minagi, Hitomi, Nohno, Tsutomu, Takabatake, Kiyofumi, Tanaka, Takehiro, Katsuyama, Takayuki, Miyawaki, Kohta, Wada, Jun, Ibaragi, Soichiro, Iida, Seiji, Yoshino, Tadashi, Nagatsuka, Hitoshi, Sakai, Takayoshi, Ohuchi, Hideyo
Předmět:	BIOPSY EPITHELIAL cells AUTOPHAGY RESEARCH funding ACADEMIC medical centers EXOCRINE glands RETROSPECTIVE studies FLUORESCENT antibody technique LYMPHOCYTES XEROSTOMIA GENE expression CONNECTIVE tissue diseases IMMUNOHISTOCHEMISTRY RNA AUTOIMMUNE diseases MEDICAL records ACQUISITION of data HISTOLOGICAL techniques LINGUAL frenum SJOGREN'S syndrome SALIVARY glands MOLECULAR pathology
Zdroj:	BMC Oral Health; 9/16/2024, Vol. 24 Issue 1, p1-14, 14p
Abstrakt:	Some forms of Sjögren's syndrome (SS) follow a clinical course accompanied by systemic symptoms caused by lymphocyte infiltration and proliferation in the liver, kidneys, and other organs. To better understand the clinical outcomes of SS, here we used minor salivary gland tissues from patients and examine their molecular, biological, and pathological characteristics. A retrospective study was performed, combining clinical data and formalin-fixed paraffin-embedded (FFPE) samples from female patients over 60 years of age who underwent biopsies at Okayama University Hospital. We employed direct digital RNA counting with nCounter® and multiplex immunofluorescence analysis with a PhenoCycler™ on the labial gland biopsies. We compared FFPE samples from SS patients who presented with other connective tissue diseases (secondary SS) with those from stable SS patients with symptoms restricted to the exocrine glands (primary SS). Secondary SS tissues showed enhanced epithelial damage and lymphocytic infiltration accompanied by elevated expression of autophagy marker genes in the immune cells of the labial glands. The close intercellular distance between helper T cells and B cells positive for autophagy-associated molecules suggests accelerated autophagy in these lymphocytes and potential B cell activation by helper T cells. These findings indicate that examination of FFPE samples from labial gland biopsies can be an effective tool for evaluating molecular histological differences between secondary and primary SS through multiplexed analysis of gene expression and tissue imaging. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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