| Autor: |
Burger, Gavriel, Rhodes, Lyndsay |
| Předmět: |
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| Zdroj: |
Florida Scientist; 2024, Vol. 87 Issue 3/4, p136-137, 2p |
| Abstrakt: |
Multicellular tumor spheroids (MTSs) are cells grown in aggregates to form a 3D tumor model. While 20 cell culture-cells grown in a monolayer-is useful for cancer research and drug screening, the response is often variable from what would occur in vivo. 3D cell culture mimics the tumor micro-environment and contains other similarities to in vivo tumors, such as necrotic and hypoxic regions, allowing for a more realistic prediction of treatment response. In this study, the protocol for generating MTSs from multiple triple-negative breast cancer (TNBC) cell lines was developed to use as a drug screening tool for potential treatments. For each cell line, an MTS model was developed and characterized to determine the cell number required to achieve spheroids with a diameter of -400 microns after 72 hours of growth. All spheroids were generated in low-adhesion cell culture plates. Developed spheroids were then tested in drug screening assays, and results were compared to previous 2D results. Data collection included final spheroid diameter, cell density, and cell viability count to assess the effects of administered treatment. This model will be used in future studies to better understand the impacts ofdrug treatments in a inore translational model. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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