| Autor: |
Bach, Adam J., Palmer, Andrew G., Brown, Alan B. |
| Předmět: |
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| Zdroj: |
Florida Scientist; 2024, Vol. 87 Issue 3/4, p108-108, 1/3p |
| Abstrakt: |
Numerous species of prokaryotes have been found to regulate symbiotic and pathogenic relationships, exhibit phenotypic switching based on cell density, and other chemical processes (i.e., metabolism) using a method known as quorum sensing. In Gram-negative bacteria quorum sensing systems allow bacteria to coordinate in specific behaviors using N-acyl L-homoserine lactones (AHLs) as a signal. AHLs are made up of an L-homoserine head group, and a varying length acyl tail and varying substituents dependent on what species of bacteria is using them to communicate. In this experiment all AHLs will be synthesized, but in their natural environment they can also be used as a nutrient source for the bacteria producing them, as well as other organisms in the surrounding microbiota. One eukaryotic organism shown to use AHLs as a nutrient source is Chlaniydomonas reinhardtii. Being a model system, C reinhardtii has been shown to secrete quorum sensing regulatory compounds that may be able to be impacted by AHLs. Due to these factors, it can be hypothesized that C. reinhardtii should be able to metabolize and incorporate the AHLs. Using Carbon-13 (130 nuclear magnetic resonance (NMR), the metabolic activity of C. reinhardrii can be traced. By feeding a 13C enriched sample of AHLs to C. reinhardtii, it will be clearly seen whether or not the AHLs can be metabolized by C reinhardtii or not due to the presence of the isotope. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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