Qualitative and Quantitative Analyses of Sialyl O -Glycans in Milk-Derived Sialylglycopeptide Concentrate.

Autor: Higuchi, Junichi, Kurogochi, Masaki, Yamaguchi, Toshiyuki, Fujio, Noriki, Mitsuduka, Sho, Ishida, Yuko, Fukudome, Hirofumi, Nonoyama, Noriko, Gota, Masayuki, Mizuno, Mamoru, Sakai, Fumihiko
Předmět:
Zdroj: Foods; Sep2024, Vol. 13 Issue 17, p2792, 14p
Abstrakt: Sialyl glycans have several biological functions. We have previously reported on the preparation and bifidogenic activity of milk-derived sialylglycopeptide (MSGP) concentrate containing sialyl O-glycans. The current study qualitatively and quantitatively analyzed the sialyl O-glycans present in the MSGP concentrate. Notably, our quantitative analysis indicated that a majority of O-glycopeptides in the MSGP concentrate were derived from glycomacropeptides. The concentrate was found to contain mainly three types of sialyl core 1 O-glycans, with the disialyl core 1 O-glycan being the most abundant. We successfully quantified three types of sialyl core 1 O-glycans using a meticulous method that used homogeneous O-glycopeptides as calibration standards. Our results provide valuable insights into assessment strategies for the quality control of O-glycans in dietary products and underscore the potential applications of MSGP concentrate in the food industry and other industries. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index