A monomeric StayGold fluorescent protein.

Autor: Ivorra-Molla, Esther, Akhuli, Dipayan, McAndrew, Martin B. L., Scott, William, Kumar, Lokesh, Palani, Saravanan, Mishima, Masanori, Crow, Allister, Balasubramanian, Mohan K.
Zdroj: Nature Biotechnology; Sep2024, Vol. 42 Issue 9, p1368-1371, 4p
Abstrakt: StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric. The bright, photostable fluorescent protein StayGold is converted into a monomer. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index