Unraveling the Microbial Diversity of Ecosystems under the influence of Environmental Pollution.

Autor: Esquilin, Elik, Laberge, Tammy, Nieves, Analuz, Valiente, Jacqueline
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Zdroj: Southeastern Biology; Jan-Dec2024, Vol. 71 Issue 1-4, p103-103, 1/3p
Abstrakt: Escalating pollution levels continue to inflict critical threats to the delicate balance of ecosystems worldwide, including those in South Florida. Investigating the repercussions of pollution on bacterial diversity within these waterways is paramount for devising effective strategies to combat its adverse effects. This study aims to shed light on the impact of pollution on bacterial biodiversity by analyzing environmental DNA (eDNA) extracted from water samples collected from polluted and unpolluted South Florida sites. Using advanced DNeasy PowerWater DNA extraction kits, DNA sequencing, and DNA Metabarcoding via DNA Subway, this research aims to identify distinct bacterial species from various locations and highlight significant differences in bacterial diversity and composition. Although the project and its findings are still ongoing, our current results reveal an overall impressive diversity of bacteria from all samples as a whole, with a total of 4125 DNA fragments identified. This quantity of diversity is significant, as it provides a robust dataset for our ongoing analyses. Further, our research aims to continue identifying specific trends in bacterial abundance and distribution from the separated polluted and unpolluted sites and potentially pinpoint key indicator species that could serve as markers for pollution levels and environmental health. For instance, we have identified a bacteria commonly found in bioremediation processes in previously restored superfund sites. By comprehending the intricate interplay between pollution and bacterial biodiversity, this study hopes to contribute to the development of proactive measures aimed at mitigating the proliferation of harmful contaminants, safeguarding the integrity of South Florida's waterways, and preserving the region's diverse ecosystems. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index