The production of medium-co-long chain length (mcl-co-lcl) polyhydroxyalkanoate from waste soybean oil by Enterobacter sp. and its application as an antibacterial agent.

Autor: Petpheng, Boossayarat, Mudtaleb, Bookree, Thongduang, Siwakorn, Meekhai, Teerawat, Pechsiri, Jamjun, Sangkharak, Kanokphorn
Zdroj: Biomass Conversion & Biorefinery; Sep2024, Vol. 14 Issue 18, p23059-23072, 14p
Abstrakt: This study evaluated the possibility of waste soybean oil (WSO) as a substrate for medium-co-long chain length (mcl-co-lcl) polyhydroxyalkanoate (PHA). In addition, the antibacterial activities of mcl-co-lcl PHA supplemented with lime essential oil were also demonstrated. High polyunsaturated fatty acids (PUFAs) (53.9%) with linoleic acid as the major fatty acid were observed in WSO. Therefore, WSO was used as a substrate for PHA production by Enterobacter TS1L. PHA production was compared between batch and fed-batch fermentation. The highest biomass (5.0 ± 0.2 g/L) and PHA (54.00% CDM, 0.028 g/L·h) were obtained at cycle 3 of cyclic fed-batch fermentation (CFBF). The biomass and PHA from CFBF were 1.8- and 1.9-fold higher than those from batch fermentation. Afterward, the polymer was characterized to be mcl-co-lcl PHA containing 3-hydroxytetradecanoate (3HTD), 3-hydroxyhexadecanoate (3HHD), and 3-hydroxyoctadecanoate (3HOD) at molar compositions of 32.44, 23.90, and 43.66, respectively. Thereafter, a film containing mcl-co-lcl PHA and lime oil was formed by casting solution, and its antibacterial activity was determined. Oil-containing PHA film exhibited a good ability against both Staphylococcus aureus and Escherichia coli. This study revealed the possibility of using WSO as a low-cost substrate to produce mcl-co-lcl PHA. In addition, the application of mcl-co-lcl PHA containing high 3HOD as an antibacterial agent has never been reported thus far. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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