| Abstrakt: |
The emerging field of biorefinery presents an opportunity to extract valuable resources from plant biomass like hemp, while tissue culture techniques, such as micropropagation, can produce uniform and high-quality plant material for biorefinery purposes. This study evaluated the use of hormonal and organic diets in creating callus from leaf fragments in three medical hemp cultivars over 8 weeks for biorefinery applications. The most effective treatment was the modified MS diet supplemented with 0.5 mg/L 2,4-D plus 0.5 mg/L KN, which induced callus formation in all three cultivars with an average size of 2.1 cm. Adding 150 mg/L of coconut water to the MS formula also showed significant results, inducing up to 50% callus formation in all three cultivars with an average size of 1.3 cm. Three different photoperiods were tested, with the 16:8 photoperiod being the most effective. White light was the most effective color, followed by blue light. The highest yield of extract was obtained from the callus section of Carmagnola hemp in the MS formula without growth regulators, with a yield of 2.75%. Consequently, this study provides valuable insights into the use of hormonal and organic diets, photoperiod, and light color for callus formation from leaf fragments in medical hemp, which could have important implications for producing high-value compounds via biorefinery processes. [ABSTRACT FROM AUTHOR] |
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