The nucleolar protein fibrillarin modulates its expression and distribution during Respiratory Sincital Virus infection.

Autor: Ulloa-Aguilar, J. M., Holguin-Cruz, V. J., Vázquez-Martínez, J. A., Herrera-Moro, Huitron L., Cedillo-Barrón, L., García-Cordero, J., Bajorek, M., Viveros-Rogel, M., Vergara-Mendoza, M., Miranda-Labra, R. U., León-Juárez, M.
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Zdroj: Veterinaria México OA; 2024 Special Issue, Vol. 11, p16-16, 1p
Abstrakt: In this work, we focus on the respiratory syncytial virus matrix protein. However,the primary function of the matrix protein is to serve as a site for viral assembly and budding at the plasma membrane. There is evidence that the matrix protein of Nipah and Hendra viruses can be transported to the nucleolus and interact with nucleolar proteins, such as fibrillarin (FBL). The interaction between FBL and matrix causes FBL to be inhibited, silencing the rRNA biogenesis. And the latter allows for an increase in the production of viral particles. In this work, we focus on evaluating the nucleolar distribution and expression of FBL protein during the infection of RSV. Our results obtained in immunofluorescence did not identify a colocalization between the matrix protein and the FBL protein. However, different distributions and proportions of this protein were observed; and performing an analysis of medium-intensity fluorescence determined a more significant signal of FBL in conditions of infection. This data was corroborated by a western blot identifying a greater expression of this nuclear protein in cells infected with RSV. In conclusion, RSV infection doesn’t exist to colocalize between FBL and matrix protein: however, it identified that RSV promotes an increase in the expression of the FBL protein. Therefore, the next step is to analyze the implication that this increase in FBL could have during RSV infection. during respiratory syncytial virus infection. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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