Autor: |
Gürbilek, Sevil Erdenliğ, Arserim, Neval Berrin, Tel, Osman Yaşar, Sertkaya, Zeynep Tugçe, Keskin, Oktay |
Předmět: |
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Zdroj: |
Indian Journal of Animal Research; Aug2024, Vol. 58 Issue 8, p1358-1361, 4p |
Abstrakt: |
Backgorund: Bacteriophages are closely related to the evolution and virulence of some important bacterial pathogens. Due to their highly significant roles in pathogenesis and virulence, S. aureus bacteriophages are frequently studied. Bacteriophages are grouped into two main categories depending on their life cycles. There are highly consistently lytic phages (virulent) and temperate phages. This study aimed to isolate bacteriophages and determine their phage serogroups from phage plaques in S. aureus cultures in order to show if they are lytic or lysogenic, the latter plays a major role in horizontal gene transfer. Methods: A total of 234 S. aureus isolates were recovered from milk samples from cases with gangrenous mastitis in sheep. Staphylococcal phages are determined based on the type and serogroup by PCR using specific primers. Result: Our study allowed us to determine serogroups of the isolated bacteriophages. Two phage stock samples included only one serogroup while the others included more than one phage serotypes and needed further purification Fa, L and D serogroups were not determined in the study. Present work revealed that all the isolated phages were temperate phages, which play a highly significant role in horizontal gene transfer. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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