Determination of Salvianolic Acid B in Yiqi Huayu Prescription by HPLC.

Autor: Yingjing WANG, Licheng SU, Yuliang HU, Zhuqiang WANG
Předmět:
Zdroj: Medicinal Plant; Feb2024, Vol. 15 Issue 1, p32-34, 3p
Abstrakt: [Objectives] To determine the content of salvianolic acid B in Yiqi Huayu Prescription by HPLC. [Methods] The chromatographic column was ZORBAX Eclipse Plus C18 (4.6 nmx250 nm, 5 μm); the mobile phase was acetonitrile-0.1% phosphoric acid (21:79), the detection wavelength was 286 nm, the column temperature was 30 °C, and the flow rate was 1.0 mL/min. A method for determination of salvianolic acid B in Yiqi Huayu Prescription was established. [Results] The linear relationship of salvianolic acid B was good in the range of 0.021 4-0.406 4 mg/mL. The regression equation was Y=5 995.989 84 X-0.073 32, r=0.999 9. The average recovery rate was 98.88% (RSD=1.6%). [Conclusions] The method is reliable, accurate and specific, and can be used for the determination of salvianolic acid B in Yiqi Huayu Prescription. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index