| Autor: |
Xiaoli Hu, Tong Li, Changbin Xu, Yanna Xu, Congjun You, Xinyi Li, Jinli Gong, Xiaolong Li, Xuepeng Sun |
| Předmět: |
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| Zdroj: |
Horticulture Plant Journal; Sep2024, Vol. 10 Issue 5, p1121-1133, 13p |
| Abstrakt: |
N6-methyladenosine (m6A) RNA modification is a conserved mechanism that regulates the fate of RNA across eukaryotic organisms. Despite its significance, a comprehensive analysis of m6A-related genes in non-model plants, such as kiwifruit, is lacking. Here, we identified 36 m6Arelated genes in the kiwifruit genome according to homology and phylogenetic inference. We performed bioinformatics and evolutionary analyses of the writer, eraser, and reader families of m6A modification. Reanalysis of public RNA-seq data collected from samples under various biotic and abiotic stresses indicated that most m6A-related genes were remarkably expressed under different conditions. Through construction of gene co-expression networks, we found significant correlations between several m6A-related genes and transcription factors (TFs) as well as receptor-like genes during the development and ripening of kiwifruit. Furthermore, we performed ATAC-seq assays on diverse kiwifruit tissues to investigate the regulatory mechanisms of m6A-related genes. We identified 10 common open chromatin regions that were present in at least two tissues, and these regions might serve as potential binding sites for MADS protein, C2H2 protein, and other predicted TFs. Our study offers comprehensive insights into the gene family of m6A-related components in kiwifruit, which will lay foundation for exploring mechanisms of post-transcriptional regulation involved in development and adaptation of kiwifruit. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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