| Autor: |
Tran Nhu, Chi, Vu Quoc, Tuan, Do Quang, Loc, Nguyen Dang, Phu, Chun-Ping, Jen, Chu Duc, Trinh, Bui Thanh, Tung |
| Předmět: |
|
| Zdroj: |
Analytical Letters; 2024, Vol. 57 Issue 17, p2959-2971, 13p |
| Abstrakt: |
This paper presents a study on the impedance spectroscopy methods applied to 2-electrode and 3-electrode biosensors. While 3-electrode electrochemical biosensors have been extensively studied and applied in various applications, the 2-electrode configuration of electrochemical biosensors still lacks comprehensive investigation for real-world applications. In particular, the miniaturization of the 3-electrode configuration for integration into microfluidic devices has been explored due to the relatively limited number of previous studies. A commercial screen-printed gold electrode was employed to develop immunosensors for bovine serum albumin (BSA) detection. The comparisons were performed on non-Faradaic and Faradic immunosensors corresponding to 2-electrode and 3-electrode configurations through impedance measurements. The results show the performance of both types of sensors in detecting BSA protein. While the 2-electrode configuration-based sensor can detect the BSA protein with a sensitivity of 12 Ω/µM, the 3-electrode configuration-based sensor shows superior results with a sensitivity of 7.2 kΩ/µM. Besides, the results also indicate that the 2-electrode configuration-based sensor can easily be miniaturized to integrate into a microfluidic channel to develop lab-on-a-chip systems. Although the 3-electrode configuration demonstrates advancements compared to the 2-electrode configuration, the 2-electrode system based on non-Faradaic processes still shows high potential for future applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
