| Autor: |
Bairwa, Aarti, Dipta, Bhawna, Siddappa, Sundaresha, Singh, Baljeet, Sharma, Neha, Naga, Kailash C., Mhatre, Priyank H., Sharma, Sanjeev, Venkatasalam, E. P., Singh, Brajesh |
| Předmět: |
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| Zdroj: |
Protoplasma; Sep2024, Vol. 261 Issue 5, p965-974, 10p |
| Abstrakt: |
Globodera pallida, an obligate sedentary endoparasite, is a major economic pest that causes substantial potato yield losses. This research aimed to study the effects of gene silencing of three FMRFamide-like peptides (FLPs) genes to reduce G. pallida infestation on potato plants by using kaolinite nanoclay as a carrier to deliver dsRNAs via drenching. A dsRNA dosage of 2.0 mg/ml silenced flp-32c by 89.5%, flp-32p by 94.6%, and flp-2 by 94.3%. J2s incubated for 5 and 10 h showed no phenotypic changes. However, J2s of G. pallida efficiently uptake dsRNA of all targeted genes after 15 h of incubation. On the other hand, J2s that had been kept for 24 h had a rigid and straight appearance. Under fluorescence microscopy, all dsRNA-treated nematodes showed fluorescein isothiocyanate (FITC) signals in the mouth, nervous system, and digestive system. The untreated population of J2s did not show any FITC signals and was mobile as usual. The drenching of potato cultivar Kufri Jyoti with the dsRNA-kaolinite formulations induced deformation and premature death of J2s, compared with untreated J2s that entered J3 or J4 stages. This study validates that the nanocarrier-delivered RNAi system could be employed effectively to manage G. pallida infestations. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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