A gap-free genome assembly of Fusarium oxysporum f. sp. conglutinans, a vascular wilt pathogen.

Autor: Wang, Huan, Yao, Gang, Chen, Weikai, Ayhan, Dilay Hazal, Wang, Xiangfeng, Sun, Jie, Yi, Shu, Meng, Tan, Chen, Shaoying, Geng, Xin, Meng, Dian, Zhang, Lili, Guo, Li
Předmět:
Zdroj: Scientific Data; 8/27/2024, Vol. 11 Issue 1, p1-10, 10p
Abstrakt: Fusarium oxysporum is an asexual filamentous fungus that causes vascular wilt in hundreds of crop plants and poses a threat to public health through Fusariosis. F. oxysporum f. sp. conglutinans strain Fo5176, originally isolated from Brassica oleracea, is pathogenic to Arabidopsis, making it a model pathosystem for dissecting the molecular mechanisms underlying host-pathogen interactions. Assembling the F. oxysporum genome is notoriously challenging due to the presence of repeat-rich accessory chromosomes. Here, we report a gap-free genome assembly of Fo5176 using PacBio HiFi and Hi-C data. The 69.56 Mb assembly contained 18 complete chromosomes, including all centromeres and most telomeres (20/36), representing the first gap-free genome sequence of a pathogenic F. oxysporum strain. In total, 21,460 protein-coding genes were annotated, a 26.3% increase compared to the most recent assembly. This high-quality reference genome for F. oxysporum f. sp. conglutinans Fo5176 provides a valuable resource for further research into fungal pathobiology and evolution. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index