| Autor: |
García-López, Laura Lorena, Vargas-Montes, Mónica, Osorio-Méndez, Juan Felipe, Cardona, Néstor, Hernández De Los Ríos, Alejandro, Toro-Acevedo, Carlos Andrés, Arenas-García, Juan Camilo, Mantilla-Muriel, Luz Eliana, Torres, Elizabeth, Valencia-Hernández, Juan D., Acosta-Dávila, Alejandro, de-la-Torre, Alejandra, Celis-Giraldo, Daniel, Mejía Oquendo, Manuela, Sepúlveda-Arias, Juan Carlos, Gómez-Marín, Jorge Enrique |
| Předmět: |
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| Zdroj: |
Ocular Immunology & Inflammation; Sep2024, Vol. 32 Issue 7, p1218-1227, 10p |
| Abstrakt: |
This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis (n = 9), chronic asymptomatic toxoplasmosis (n = 7), and non-infected people (n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals (p =.003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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