| Autor: |
Edwards, Natasha, Reboud, Julien, Xiaoxiang Yan, Xin Guo, Cooper, Jonathan M., Wadsworth, Jemma, Waters, Ryan, Mioulet, Valerie, King, Donald P., Shaw, Andrew E. |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology; 2024, p1-9, 9p |
| Abstrakt: |
Foot-and-mouth disease (FMD) is a highly contagious viral disease of clovenhoofed animals responsible for economic losses that amount to >$20 billion annually. Rapid recognition of FMD cases provides vital information to guide control programmes. A range of point-of-need amplification technologies have been developed which allow sensitive detection of the causative virus (FMDV) in the field at locations remote from laboratories. Here we describe a novel system to detect FMDV RNA using loop-mediated isothermal amplification (LAMP). This test was evaluated using a panel of FMDV isolates (n = 79) and RNA standards demonstrating capability to amplify viral genome directly from clinical material in the absence of nucleic acid extraction. This extraction-free RT-LAMP assay was transferred to a bespoke closed-system lateral flow test (LFT) that was used in combination with a low-cost hand-held heater. Our results show that the RT-LAMP-LFT assay retains a high level of diagnostic and analytical sensitivity when using direct clinical material, with a limit of detection under 80 copies per reaction. Together, our data support the potential for the use of this assay at the point-of-need to facilitate rapid feedback on the status of suspect cases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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