| Autor: |
Mohammed, Mubasher, Dziedziech, Alexis, Macedo, Diego, Huppertz, Frederik, Veith, Ylva, Postel, Zoé, Christ, Elena, Scheytt, Richard, Slotte, Tanja, Henriksson, Johan, Ankarklev, Johan |
| Předmět: |
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| Zdroj: |
Nature Communications; 8/26/2024, Vol. 15 Issue 1, p1-20, 20p |
| Abstrakt: |
The Plasmodium falciparum life cycle includes obligate transition between a human and mosquito host. Gametocytes are responsible for transmission from the human to the mosquito vector where gamete fusion followed by meiosis occurs. To elucidate how male and female gametocytes differentiate in the absence of sex chromosomes, we perform FACS-based cell enrichment of a P. falciparum gametocyte reporter line followed by single-cell RNA-seq. In our analyses we define the transcriptional programs and predict candidate driver genes underlying male and female development, including genes from the ApiAP2 family of transcription factors. A motif-driven, gene regulatory network analysis indicates that AP2-G5 specifically modulates male development. Additionally, genes linked to the inner membrane complex, involved in morphological changes, are uniquely expressed in the female lineage. The transcriptional programs of male and female development detailed herein allow for further exploration of the evolution of sex in eukaryotes and provide targets for future development of transmission blocking therapies. P. falciparum (malaria) transmission includes the development of male and female parasite forms called gametocytes, which are taken up by the Anopheles mosquito. The authors of this study use single cell transcriptomics to define the transcriptional programs, identify key regulators and predict novel genes, involved in driving the male and female sexual cell fates. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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