| Autor: |
Cui, Guangjie, Kim, Do Young, Zu, Di, Chai, Guanbo, Lee, Somin Eunice |
| Zdroj: |
ACS Applied Nano Materials; 8/23/2024, Vol. 7 Issue 16, p18443-18449, 7p |
| Abstrakt: |
The ability to spatially and temporally map nanoscale environments in situ over extended time scales would be transformative for biology, biomedicine, and bioengineering. All nanometer objects, from nanoparticles down to single proteins, scatter light. Interferometric scattering stands as a powerful tool, offering ultrasensitivity and resolution vital for visualizing nanoscale entities. Interferometric scattering from an individual nanoparticle down to an individual protein has been detected; however, resolving adjacent nanometer objects with interferometric scattering has not yet been demonstrated. In this work, we present interferometric phase intensity nanoscopy (iPINE) to resolve adjacent nanometer objects with interferometric scattering. We demonstrate that multiphase and sensitivity of iPINE reveal ellipse Airy patterns correlated with nanostructural features. We show that eliminating background fluctuation by employing circularly polarized illumination in iPINE is essential to separate proximal nanometer objects below the diffraction limit. We envision iPINE for resolving a variety of adjacent nanometer objects from nanoparticles down to proximal proteins in situ over extended time periods for a wide range of applications in biology, biomedicine, and bioengineering. We expect iPINE to be especially important in applications of biological dynamics that require extended observation times. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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