Phytochemical and biological investigation of Astragalus Caprinus L.
| Autor: | Abdallah, Walid E., Abdelshafeek, Khaled A., Elsayed, Wael M., AbdelMohsen, Mona M., Salah, Neven A., Hassanein, Heba D. |
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| Předmět: |
ORGANIC compound analysis
ASTRAGALUS (Plants) GRAM-positive bacterial infections FLAVONOIDS ANTIMICROBIAL stewardship PHYTOCHEMICALS PLANT roots STAPHYLOCOCCUS aureus DESCRIPTIVE statistics PLANT extracts CELL lines GAS chromatography CANDIDA albicans METABOLITES ANTI-infective agents MEDICINAL plants MASS spectrometry BACTERIAL diseases ETHERS FATTY acids GRAM-negative bacterial diseases DATA analysis software PSEUDOMONAS KLEBSIELLA |
| Zdroj: | BMC Complementary Medicine & Therapies; 8/22/2024, Vol. 24 Issue 1, p1-12, 12p |
| Abstrakt: | Background: cultivated and wild plants are used to treat different ailments. The Astragalus genus is found in temperate and dry climates; thus, it is found in Egypt and the arab world. Astragalus caprinus has a good amount of bioactive chemicals, which may help explain its therapeutic effects in reducing the risk of consequences from disease. Method: The phytochemical investigation of the herb and roots of Astragalus caprinus L. included the analytical characterization for the petroleum ether components by GC/MS, unsaponifiable matter (unsap. fraction), and fatty acids (FAME) investigation by GLC analysis. Main flavonoids were chromatographically isolated from ethyl acetate and n-butanol extracts. In vitro antimicrobial activity has been tested against the Gram-positive bacteria Staphylococcus aureus and Streptococcus mutans for different plant extracts, the Gram-negative bacteria Pseudomonas aeruginosa and Klebsiella pneumonia, the fungus Candida albicans and Aspergillus niger, and the Escherichia coli bacterium. Metabolite cytotoxicity was examined using the MTT assay against HepG-2 (human liver carcinoma) and MCF-7 (breast carcinoma). Results: Identifying the important components of the herb and root petroleum ether extracts was achieved. Using column chromatography, luteolin, cosmosiin (apigenin-7-O-glucoside), and cynaroside (luteolin-7-O-glucoside) were separated and identified using UV, NMR, and Mass Spectroscopy. Root extracts displayed potential antimicrobial activity against most of the tested pathogens. Both extracts (herb and roots) were active against the MCF-7 cell line and HepG-2 cell line with IC |
| Databáze: | Complementary Index |
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