Visual detection of biogenic monoamines based on amine oxidase–peroxidase coupling reaction using ascorbic acid as H2O2 scavenger.

Autor: Kim, Young-Chang, Ha, Jun-Mo, Lee, Jaeick, Kim, Young-Wan
Zdroj: Food Science & Biotechnology; Sep2024, Vol. 33 Issue 12, p2755-2760, 6p
Abstrakt: This study represents a visual detection for total biogenic monoamines with naked eye as a simple and rapid semi-quantitative method for biogenic amine monitoring. The equivalent reaction of H2O2 with ascorbic acid resulted in color development by an amine oxidase–peroxidase coupling reaction in the samples containing the biogenic monoamines higher than the subjected ascorbic acid by 10 μM. Upon employing the commercial doenjang extracts as a model food, an additional heating step was requested, and the expected ranges for the biogenic monoamines from 360 to 480 μM covered the real contents of the samples (360.2–407.3 μM). Therefore, this visual detection method makes it possible to decide with naked eye whether the sample contains the biogenic monoamines higher than the ascorbic acid supplemented as much as a control level on manufacturing sites without instrumental analysis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index