Ginseng-DF ameliorates intestinal mucosal barrier injury and enhances immunity in immunosuppressed mice by regulating MAPK/NF-κB signaling pathways.
| Autor: | Sha, Ji-yue, Chen, Ke-Cheng, Liu, Zheng-bo, Li, Wei, Lu, Yu-shun, Liu, Shuang, Ma, Jian-kai, Qu, Di, Sun, Yin-shi |
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| Předmět: |
INTESTINAL mucosa injuries
MITOGEN-activated protein kinases NF-kappa B FLOW cytometry EPITHELIAL cells RESEARCH funding T cells IMMUNOGLOBULINS APOPTOSIS CELLULAR signal transduction INTESTINAL diseases MICE ANIMAL experimentation WESTERN immunoblotting GINSENG BIOLOGICAL assay IMMUNITY IMMUNOSUPPRESSION CYCLOPHOSPHAMIDE TUMOR necrosis factors INTERLEUKINS |
| Zdroj: | European Journal of Nutrition; Aug2024, Vol. 63 Issue 5, p1487-1500, 14p |
| Abstrakt: | Purpose: Dietary fiber (DF) has a good application prospect in effectively restoring the integrity of the intestinal mucosal barrier. Ginseng-DF has good physicochemical properties and physiological activity and shows positive effects in enhancing immunity. The aim of this study was to investigate the protective effect of Ginseng-DF on intestinal mucosal barrier injury induced by cyclophosphamide (CTX) in immunosuppressed mice and its possible mechanism. Methods: The effects of Gginseng-DF on immune function in mice were studied by delayed-type hypersensitivy, lymphocyte proliferation assay and NK cytotoxicity assay, the T lymphocyte differentiation and intestinal barrier integrity were analyzed by flow cytometry and western blot. Results: Ginseng-DF (2.5% and 5%) could attenuate the inhibition of DTH response by CTX, promote the transformation and proliferation of lymphocytes, and stimulate NK effector cell activity. At the same time, Ginseng-DF could restore the proportion of CD4+/CD8+ T lymphocytes induced by CTX to different extents, improved spleen tissue damage, promoted the secretion of immunoglobulin IgG, and enhanced body immunity. More importantly, Ginseng-DF could up-regulate the contents of TNF-α, IFN-γ, IL-6 and IL-1β in serum and intestine of immunosuppressed mice to maintain the balance between Th1/Th2 cytokines, and improve the permeability of intestinal mucosal barrier. Meanwhile, Ginseng-DF could reduce intestinal epithelial cell apoptosis and improve intestinal adaptive immunity in CTX-induced immunosuppressed mice by regulating MAPK/NF-κB signaling pathway. Conclusion: Ginseng-DF can be used as a safe dietary supplement to enhance body immunity and reduce intestinal mucosal injury caused by CTX. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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