| Autor: |
Huynh, Julie, Nhat, Le Hoang Thanh, Bao, Nguyen Le Hoai, Hai, Hoang Thanh, Thu, Do Dang Anh, Tram, Trinh Thi Bich, Dung, Vu Thi Mong, Vinh, Do Dinh, Ngoc, Nghiem My, Donovan, Joseph, Phu, Nguyen Hoan, Thanh, Dang Van, Thu, Nguyen Thi Anh, Bang, Nguyen Duc, Ha, Dang Thi Minh, Nghia, Ho Dang Trung, Tan, Le Van, Van, Le Hong, Thwaites, Guy, Thuong, Nguyen Thuy Thuong |
| Předmět: |
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| Zdroj: |
Journal of Infectious Diseases; 8/15/2024, Vol. 230 Issue 2, pe268-e278, 11p |
| Abstrakt: |
Background Tuberculous meningitis (TBM) is difficult to diagnose. We investigated whether a 3-gene host response signature in blood can distinguish TBM from other brain infections. Methods The expression of 3 genes (dual specificity phosphatase 3 [ DUSP3 ], guanylate-binding protein [ GBP5 ], krupple-like factor 2 [ KLF2 ]) was analyzed by RNA sequencing of archived whole blood from 4 cohorts of Vietnamese adults: 281 with TBM, 279 with pulmonary tuberculosis, 50 with other brain infections, and 30 healthy controls. Tuberculosis scores (combined 3-gene expression) were calculated following published methodology and discriminatory performance compared using area under a receiver operator characteristic curve (AUC). Results GBP5 was upregulated in TBM compared to other brain infections (P <.001), with no difference in DUSP3 and KLF2 expression. The diagnostic performance of GBP5 alone (AUC, 0.74; 95% confidence interval [CI],.67–.81) was slightly better than the 3-gene tuberculosis score (AUC, 0.66; 95% CI,.58–.73) in TBM. Both GBP5 expression and tuberculosis score were higher in participants with human immunodeficiency virus (HIV; P <.001), with good diagnostic performance of GBP5 alone (AUC, 0.86; 95% CI,.80–.93). Conclusions The 3-gene host signature in whole blood has the ability to discriminate TBM from other brain infections, including in individuals with HIV. Validation in large prospective diagnostic study is now required. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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