| Autor: |
Stranak, Zbynek, Ardan, Taras, Nemesh, Yaroslav, Toms, Maria, Toualbi, Lyes, Harbottle, Richard, Ellederova, Zdenka, Lytvynchuk, Lyubomyr, Petrovski, Goran, Motlik, Jan, Moosajee, Mariya, Kozak, Igor |
| Předmět: |
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| Zdroj: |
Current Eye Research; Aug2024, Vol. 49 Issue 8, p879-887, 9p |
| Abstrakt: |
Purpose: To assess the safety and feasibility of direct vitrectomy-sparing subretinal injection for gene delivery in a large animal model. Methods: The experimental Liběchov minipigs were used for subretinal delivery of a plasmid DNA vector (pS/MAR-CMV-copGFP) with cytomegalovirus (CMV) promoter, green fluorescent protein (GFP) reporter (copGFP) and a scaffold/matrix attachment region (S/MAR) sequence. The eyes were randomized to subretinal injection of the vector following pars plana vitrectomy (control group) or a direct injection without prior vitrectomy surgery (experimental group). Intra- and post-operative observations up to 30 days after surgery were compared. Results: Six eyes of three mini-pigs underwent surgery for delivery into the subretinal space. Two eyes in the control group were operated with a classical approach (lens-sparing vitrectomy and posterior hyaloid detachment). The other four eyes in the experimental group were injected directly with a subretinal cannula without vitrectomy surgery. No adverse events, such as endophthalmitis, retinal detachment and intraocular pressure elevation were observed post-operatively. The eyes in the experimental group had both shorter surgical time and recovery while achieving the same surgical goal. Conclusions: This pilot study demonstrates that successful subretinal delivery of gene therapy vectors is achievable using a direct injection without prior vitrectomy surgery. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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