| Abstrakt: |
The current investigation is aimed to evaluate the effectiveness of bovine serum albumin (BSA) polymer nanoparticles coated amentoflavone compound derived from Cassia fistula leaf extract against bacteria and cancer cells. The nanoparticles were characterized using UV, XRD, FTIR, SEM-EDX, and HR-TEM analysis. UV analysis confirmed the nanoparticle spectrum with a maximum absorbance at 285 nm. The X-ray diffraction analysis reflected the presence of significant diffraction peak ranges at (004), (112), (201), (103), (110), (012), (110), (110), and (110) planes of face-centered cubic BSA coated amentoflavone. The HR-TEM analysis expressed the presence of spherical structured particles with an average size of 37 nm. SEM-EDX results showed 46.11%, 39.74%, and 7.11% of sulfur, carbon, and oxygen respectively. The antibacterial activity of BSA-amentoflavone nanoparticles has a broad spectrum of growth inhibition against tested human pathogenic bacteria, viz., S. aureus (7.3 ± 0.2), B. cereus (6.2 ± 0.7), E. coli (7.3 ± 0.1), and K. pneumoniae (6.1 ± 0.3 mm). The in vitro cytotoxicity assay depicted significant cytotoxic effects of amentoflavone against liver cancer (HepG2) cell line and reduced cell viability by 24% after 72 h of treatment. The overall findings suggest that BSA-amentoflavone nanoparticles could serve as an alternate biomedical agent for future therapeutic applications in the pharmaceutical avenue. [ABSTRACT FROM AUTHOR] |
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