Development of a new SNP panel for bowhead whales (Balaena mysticetus).

Autor: BAIRD, AMY B., SUYDAM, ROBERT S., POSTMA, LIANNE D., GEORGES, MARY, RIMMELIN, ALESHA R., GEORGE, J. C., BICKHAM, JOHN W.
Předmět:
Zdroj: Journal of Cetacean Research & Management; 2023, Vol. 24, p13-27, 15p
Abstrakt: Population genetic research is a critical tool for the conservation and management of marine mammals and other species. The bowhead whale (Balaena mysticetus) is subject to aboriginal subsistence hunting in Alaska, Canada, Chukotka (Russian Federation) and Greenland, where, except for Canada, it is managed by the International Whaling Commission (IWC). Molecular genetic studies support conservation management plans and aid determination of sustainable hunting quotas by providing information on levels of genetic diversity, estimates of census size, effective population size and structure. Because bowhead populations are monitored, including genetic monitoring, in several countries, there is a need for genetic methods that can be consistently used in multiple labs and provide comparable data that can be publicly shared and built upon by successive studies. Here we present a new panel of single nucleotide polymorphisms (SNPs), derived from multiple bowhead populations, which meets these criteria. We describe the use of the Fluidigm SNPtype assay for analysing 69 autosomal, six X‐chromosome and one Y‐chromosome SNPs. Results indicate the methods herein have high efficacy and low error rates. Furthermore, because SNPs are discrete sequence‐based genetic markers, the panel of loci described here can be reliably replicated and is directly comparable across different labs, making this SNP panel more useful than existing microsatellite markers. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index