| Autor: |
Zhao, Gang, Liu, Wenling, Lin, Guowei, Wen, Jing |
| Předmět: |
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| Zdroj: |
Functional Plant Biology; 2024, Vol. 51 Issue 8, p1-9, 9p |
| Abstrakt: |
CONSTANS-LIKE (COL) genes are a key signalling molecule that regulates plant growth and development during the photoperiod. Our preliminary experiments showed that the photoperiod greatly influence the formation of Tetrastigma hemsleyanum root tubers. In this study, we examined the oscillation patterns and expression characteristics of COL genes in leaves of T. hemsleyanum under different photoperiod conditions. Six genes were selected as candidate reference genes for further analyses: (1) 18S ribosomal RNA (18S rRNA); (2) α-tubulin (TUBA); (3) 30S ribosomal RNA (30S rRNA); (4) TATA binding protein (TBP); (5) elongation factor 1α (EF-1α); and (6) RNA polymerase II (RPII). The geNorm, NormFinder, and BestKeeper software programs were used to evaluate expression stability. Two ThCOL genes were screened in the T. hemsleyanum transcriptome library, and their expression patterns under different photoperiod conditions were analysed using quantitative reverse transcription PCR. The genes EF-1α , TUBA , and 18S rRNA were used to analyse the expression profiles of CONSTANS genes (ThCOL4 and ThCOL5) under different photoperiods. The expression peaks of ThCOL4 and ThCOL5 appeared at different times, demonstrating that their oscillation patterns were influenced by the photoperiod. We speculate that these two ThCOL genes may be involved in different biological processes. The CONSTANS-LIKE (COL) gene is key to plant photoperiod signalling. Tetrastigma hemsleyanum is a medicinal plant that grows only in China. Its root tuber extracts have good effect on the clinical treatment of tumours. The formation and development of its tuberous roots are related to photoperiod. In this study, the expression profiles of COL genes in T. hemsleyanum leaf under different photoperiod conditions was analysed and used to analyse whether COL genes have a role in T. hemsleyanum perception of photoperiod signalling. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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